Diaphragm valves (or membrane valves) consists of a valve body with two or more ports, a diaphragm, and a "saddle" or seat upon which the diaphragm closes the valve. The valve is constructed from either plastic or steel.

Originally, the diaphragm valve was developed for use in non-hygienic applications. Later on the design was adapted for use in the bio-pharmaceutical industry by using compliant materials that can withstand sanitizing and sterilizing methods.

There are two main categories of diaphragm valves: one type seals over a "weir" (saddle) and the other (sometimes called a "straight-way" valve) seals over a seat. The saddle type is the most common in process applications and the seat-type is more commonly used as a tank bottom valve but exists also as a process valve. While diaphragm valves usually come in two-port forms (2/2-way diaphragm valve), they can also come with three ports (3/2-way diaphragm valves also called t-valves) and more (so called block-valves). When more than three ports are included, they generally require more than one diaphragm seat; however, special dual actuators can handle more ports with one membrane.

Diaphragm valves can be manual or automated. Their application is generally as shut-off valves in process systems within the food and beverage, pharmaceutical and biotech industries. The older generation of these valves is not suited for regulating and controlling process flows, however newer developments in this area have successfully tackled this problem.

In addition to the well known, two way shut off diaphragm valve, other types include: three way zero deadleg valve, sterile access port, block and bleed, valbow and tank bottom valve just to name a few.